BREAST CYST CARCINOMA & METASTATIS HERBAL TNF AND APOPTOSIS BLUEPRINT THIRD TRAIL🥴

DAY 1

Monocef 1gm injection drep with Saline and mannitol for 2days

Diclofenac sodium 50 mg

Day 3

Augmentin 625mg oral BD for 5 days

Voveran sr 100 bd

DAY 8

REPEAT FOR NEXT 5DAYS

WITH

LIvoFLOXACIN 200mg bis

AND METROGYL 400MG bis

Day 15

ShatavAri roots and bark bhashma with pure silver incinerated at 450 degree celcious for 5hours  

The obtained ash value determined and recostituTe with MANNITOL STIRRED 500 RPM MIN. ADDED WITH TIN WITH 6.0 pH filtered with assembly 0.22 micron meter filter paper.

Than after the 'shatavari COLLOID' solution is freeze dried cold kit

The entity obtained labelled with technetium 99m and tin chloride and TNF banyan tree peptide resins purified in 100 ppm  or 10-75 mQ b

INJECTED on 16th day for pet and spect scanning

18DAYS

TETROPHOSPHINE COLD KIT PET SCANNING

FOR NEXT 

20DAYS

21rd Day mibi- cu petscanning

22rd day dtpa scanning

All labelled with methanol media and technecium 99m with qs maqberell

(HERBAL TREATMENT)

Vinca Rosea dried yellow leaf 2 leaves per day BD From day 15 to 30 days

DESECT THE 'CRYSTAL specimen' from affected invasion on day 31 (which may be painful)

#HarGharVinca💞🌾🎋🪾🔥⚛️

#Mars🫶🥀🤟⚛️

#HARGHARMADHUVINCA🛞🎍🌱🥴🤟🦚⚛️

#ASHVALUE🔥⚛️🪾🥴

#BREAST_CANCER🤟🏻

#THERAPEUTIC_COLDKIT

CHARITRA (BALI IN RAMAYANA) PART- I

Vali (Sanskrit: वाली) also known as Bali, was a vanara and the king of Kishkindha in the Hindu epic Ramayana. He was the son of Indra, the husband of Tara, the elder brother of Sugriva, and the father of Angada through his wife, Tara.

In the Ramayana, Rumā and Tārā are closely connected to Bāli (Vali), the mighty Vānara king of Kiṣkindhā, but their relationships with him are different in role, depth, and moral symbolism.

1. Rumā and Bāli (Vali)

Who is Rumā?

Rumā is the first and rightful wife of Bāli.

She is known for her patience, loyalty, and suffering.

Daughter of a Gandharva (in some versions), symbolizing purity and restraint.

Relationship with Bāli

Rumā and Bāli shared a legitimate marital bond.

When Bāli fought Mayāvi and was presumed dead, Sugrīva took Rumā under royal protection (as per some traditions).

After Bāli returned alive, a misunderstanding arose.

In anger, Bāli rejected Rumā and sent her away, believing she had accepted Sugrīva.

Significance

Rumā’s suffering reflects injustice caused by anger and ego.

She remains morally blameless throughout the epic.

Her pain is one of the causes of the rift between Bāli and Sugrīva.

2. Tārā and Bāli (Vali)

Who is Tārā?

Tārā is Bāli’s chief queen and the most intelligent female character among the Vānara women.

She is wise, diplomatic, and well-versed in dharma and politics.

Relationship with Bāli

Tārā is deeply loved and respected by Bāli.

She warns Bāli not to fight Sugrīva after realizing Rāma’s involvement.

Despite her correct advice, Bāli ignores her counsel.

After Bāli’s death

Following Bāli’s death at Rāma’s hands, Tārā marries Sugrīva.

This marriage was:

Political (to stabilize Kiṣkindhā),

Socially accepted in Vānara customs,

Focused on the welfare of Angada (her son).

3. Rumā vs Tārā – Moral Contrast

Aspect

Rumā

Tārā

Status

First wife

Chief queen

Nature

Silent suffering, devotion

Wisdom, diplomacy

Role

Moral victim

Moral advisor

Symbolism

Endurance of injustice

Voice of reason

After Bāli

Reunited with Sugrīva (in some versions)

Marries Sugrīva

4. Deeper Dhārmic Meaning

Rumā represents the pain of neglected righteousness.

Tārā represents intellect ignored by power.

Bāli symbolizes strength without restraint, which leads to downfall.

Their relationships show that physical power without dharma destroys family and kingdom.

One-line essence:

Bāli loved Tārā’s wisdom but did not follow it; he doubted Rumā’s purity and lost his righteousness—this dual failure led to his fall.

चरित्र (CHARITRA) क्या है?

चरित्र मनुष्य के विचार, आचरण, कर्म, संयम, नैतिकता और आत्मशुद्धि का समग्र स्वरूप है।

जैसे शरीर में अच्छी और संक्रमित कोशिकाएँ होती हैं, वैसे ही जीवन में शुद्ध और अशुद्ध कर्म होते हैं।

इन कर्मों का संचय ही चरित्र कहलाता है।

चरित्र = शरीर + मन + आत्मा का स्वास्थ्य

शुद्ध चरित्र → सकारात्मक ऊर्जा, मानसिक शांति, रोगों से लड़ने की शक्ति

दूषित चरित्र → अपराधबोध, मानसिक तनाव, आत्मग्लानि, रोगों की संभावना

भारतीय दर्शन में माना गया है कि

मन के विकार शरीर में रोग बनकर प्रकट होते हैं।

अग्नि समाधि (दाह संस्कार) और चरित्र

केवल शरीर का दहन (Fire Samadhi)

➡️ कर्मों को नष्ट नहीं करता

यदि जीवन में चरित्र अशुद्ध रहा है, तो

कर्मों का फल भोगना पड़ता है

पीड़ा, रोग और कष्ट जीवन में आते हैं

गीता में कहा गया है:

“अवश्यं भावी कर्मणां भोगो भवति।”

(कर्मों का फल अवश्य भोगना पड़ता है)

चरित्र कैसे दूषित होता है?

नीचे बताए गए कर्म चरित्र के खाते (Moral Ledger) में नकारात्मक अंक जोड़ते हैं:

1. वैवाहिक विश्वासघात

विवाह के बाद भी

अवैध संबंध

परस्त्री/परपुरुष गमन

➡️ यह चरित्र का सबसे गंभीर दोष माना गया है

2. नशा और आत्मसंयम का अभाव

धूम्रपान

शराब

हुक्का

नशीले पदार्थ (ड्रग्स)

➡️ ये कर्म:

मस्तिष्क को कमजोर करते हैं

विवेक नष्ट करते हैं

आत्मा को जड़ बनाते हैं

3. कामवासनात्मक अनियंत्रण

अत्यधिक हस्तमैथुन

वेश्यावृत्ति

अस्वाभाविक यौन आचरण

➡️ इससे:

मानसिक असंतुलन

ऊर्जा क्षय

आत्मगौरव का पतन होता है

4. बहुविवाह और अस्थिर संबंध

बिना नैतिक जिम्मेदारी के अनेक संबंध

➡️ यह मन को चंचल और आत्मा को अशांत करता है

5. भोजन और व्यापार में अधर्म

मिलावटी खाद्य पदार्थ खरीदना/बेचना

जानबूझकर अशुद्ध भोजन

केवल लालच से व्यापार

➡️ अन्न = मन

➡️ अशुद्ध अन्न = अशुद्ध विचार

चरित्र और रोग (आध्यात्मिक दृष्टि से)

यह मान्यता है कि:

दीर्घकालिक अपराधबोध

अपराधपूर्ण जीवन

आत्मग्लानि

➡️ शरीर की रोग-प्रतिरोधक शक्ति को कमजोर करते हैं

➡️ गंभीर रोग लंबे समय तक टिकते हैं

शुद्ध चरित्र वाले व्यक्ति के साथ क्या होता है?

रोग आता है, पर ठहरता नहीं

उपचार शीघ्र असर करता है

मानसिक शक्ति रोग से लड़ती है

पूर्ण स्वस्थ होने की संभावना अधिक होती है

इसीलिए कहा गया है:

“चरित्र ही सबसे बड़ा कवच है।”

सारांश (निष्कर्ष)

चरित्र कोई एक कर्म नहीं, बल्कि पूरा जीवन-पथ है

हर गलत कर्म चरित्र में जुड़ता है

हर सही कर्म चरित्र को शुद्ध करता है

शुद्ध चरित्र = तेजस्वी जीवन

दूषित चरित्र = कष्टमय जीवन

#HarGharVinca✨🌱

#sushane_vaidya✨🙏🏻

VINCA ROSEAE (SADABAHAR) AND ITS ENDOPHYTES TO PRODUCE VACCINE CALLED VINCA VACCINE WHICH IMMUNISED AND TO ERADICATE LIFE THREATENING DISEASE FOR MAN KIND

नीचे VINCA ROSEA (सदाबहार) से प्राप्त एंडोफाइट-आधारित “Vinca Vaccine” की अवधारणा को शोध/थीसिस-शैली में, विस्तृत लेकिन वैचारिक (conceptual) रूप में हिंदी में समझाया गया है। इसमें प्रयोगात्मक चरणों का व्यावहारिक निर्देश नहीं, बल्कि वैज्ञानिक सिद्धांत और तर्क प्रस्तुत किए गए हैं, जो अकादमिक लेखन के लिए उपयुक्त हैं।

सदाबहार (Vinca rosea) के एंडोफाइट्स से विकसित “Vinca Vaccine” : सैद्धांतिक व्याख्या

1. भूमिका (Introduction)

Vinca rosea (सदाबहार) एक सुप्रसिद्ध औषधीय पौधा है, जिसके भीतर (ऊतक के अंदर) पाए जाने वाले सूक्ष्मजीवों को एंडोफाइट्स कहा जाता है। ये एंडोफाइट्स पौधे के साथ सहजीवी संबंध में रहते हैं और अनेक जैव-सक्रिय (bioactive) अणुओं का उत्पादन करते हैं। आधुनिक जैव-प्रौद्योगिकी में यह माना जाता है कि ऐसे एंडोफाइट्स से प्राप्त जैविक घटक प्रतिरक्षा प्रणाली (immune system) को सक्रिय या प्रशिक्षित करने की क्षमता रखते हैं। इसी सैद्धांतिक आधार पर “Vinca Vaccine” की अवधारणा प्रस्तुत की जाती है।

2. एंडोफाइट्स का स्रोत (Source of Endophytes)

सदाबहार पौधे के

पत्तियों के निचले भाग (beneath the leaf) तथा

जड़ों (root tissues)

में पाए जाने वाले एंडोफाइट्स को चुना जाता है।

इन क्षेत्रों में उपस्थित एंडोफाइट्स विशेष रूप से महत्वपूर्ण माने जाते हैं क्योंकि वे पौधे की रक्षा, द्वितीयक चयापचय (secondary metabolism) और रोग-प्रतिरोधक यौगिकों के निर्माण से जुड़े होते हैं।

3. निष्कर्षण एवं किण्वन की अवधारणा (Extraction & Fermentation – Conceptual)

(i) निष्कर्षण (Extraction)

निष्कर्षण का उद्देश्य पौधे के ऊतकों के भीतर उपस्थित एंडोफाइट्स अथवा उनके जैविक उत्पादों को अलग करना होता है। यह प्रक्रिया कोशिकीय संरचना को समझने और एंडोफाइट-उत्पन्न अणुओं की पहचान के लिए सैद्धांतिक रूप से महत्वपूर्ण है।

(ii) किण्वन (Fermentation)

किण्वन को एक ऐसी जैविक अवस्था के रूप में देखा जाता है जिसमें एंडोफाइट्स अपने चयापचय उत्पादों को अधिक मात्रा में व्यक्त करते हैं। यह चरण इस विचार को समर्थन देता है कि एंडोफाइट्स से प्राप्त अणु प्रतिरक्षा-उत्तेजक (immunostimulatory) हो सकते हैं।

4. उपयुक्त माध्यम में संवर्धन की सैद्धांतिक भूमिका

Fluid Thioglycollate Medium जैसे माध्यम का उल्लेख एक सामान्य जैव-संस्कृति अवधारणा के रूप में किया जाता है, जहाँ एंडोफाइट्स को नियंत्रित वातावरण में अध्ययन हेतु रखा जा सकता है।

यह माध्यम ऑक्सीजन-संवेदनशील सूक्ष्मजीवों के अध्ययन के लिए जाना जाता है, जिससे एंडोफाइट जीनोम और उनके जैविक व्यवहार को समझने में सहायता मिलती है।

5. सैंपल-आधारित प्रतिरक्षा अवधारणा

Sample 1 (Direct Sample)

एंडोफाइट्स का जीनोमिक या जैविक अंश

इसे प्रतिरक्षा-अध्ययन के लिए प्रत्यक्ष जैविक सिग्नल के रूप में देखा जाता है

इसका उद्देश्य यह समझना है कि एंडोफाइट-उत्पन्न अणु प्रतिरक्षा तंत्र को कैसे पहचान संकेत (immune recognition signals) प्रदान करते हैं

Sample 2 (Indirect Sample)

स्वस्थ पशु मॉडल (जैसे घोड़ा) में प्रतिरक्षा प्रतिक्रिया के बाद प्राप्त एंटीबॉडी-समृद्ध जैविक अंश

यह अप्रत्यक्ष प्रतिरक्षा स्मृति (immune memory) का प्रतिनिधित्व करता है

इससे यह अध्ययन किया जाता है कि शरीर किस प्रकार एंडोफाइट-जनित एंटीजन के विरुद्ध एंटीबॉडी बनाता है

इस प्रकार Sample 1 एंटीजन-आधारित अवधारणा और Sample 2 एंटीबॉडी-आधारित अवधारणा को दर्शाता है।

6. बफर सिस्टम की भूमिका (Role of Buffer Solutions)

Disodium hydrogen phosphate, Sodium dihydrogen phosphate अथवा Sodium acetate जैसे बफर घोलों का उपयोग pH 5–7.5 की सीमा में किया जाता है।

सैद्धांतिक रूप से इन बफर्स का महत्व निम्नलिखित है:

जैविक अणुओं की स्थिरता (stability) बनाए रखना

प्रोटीन, एंटीजन या एंटीबॉडी की संरचनात्मक अखंडता को सुरक्षित रखना

प्रतिरक्षा-सक्रिय अणुओं को निष्क्रिय होने से बचाना

7. “Vinca Vaccine” की सैद्धांतिक परिकल्पना (Hypothesis)

यह परिकल्पना इस विचार पर आधारित है कि:

सदाबहार के एंडोफाइट्स प्राकृतिक प्रतिरक्षा-संशोधक हो सकते हैं

इनके जैविक उत्पाद शरीर की इम्यून सर्विलांस प्रणाली को प्रशिक्षित कर सकते हैं

प्रत्यक्ष (Sample 1) और अप्रत्यक्ष (Sample 2) दोनों दृष्टिकोण मिलकर रोग-निरोधक या सहायक प्रतिरक्षा रणनीति का आधार बना सकते हैं

8. निष्कर्ष (Conclusion)

“Vinca Vaccine” एक एंडोफाइट-आधारित वैचारिक जैव-प्रतिरक्षा मॉडल है, जो औषधीय पौधों, सूक्ष्मजीवों और प्रतिरक्षा विज्ञान के संगम को दर्शाता है। यह अवधारणा पारंपरिक औषधीय ज्ञान और आधुनिक जैव-विज्ञान के बीच सेतु बनाती है तथा भविष्य में प्लांट-माइक्रोब-इम्यून इंटरैक्शन पर आधारित अनुसंधान की दिशा सुझाती है।

यदि आप चाहें तो मैं इसे

PhD/MD थीसिस के अध्याय-रूप,

फ्लो-डायग्राम,

या शुद्ध वैज्ञानिक हिंदी (कम आध्यात्मिक, अधिक अकादमिक) शैली में भी पुनर्लेखित कर सकता हूँ।

CHARITRA (IN MAHABHARATA DRAUPADI) – PART II THE SMILE AND CHARM OF VINCA ROSEA✨🌱

 CHARITRA (चरित्र) across Yugas, using the framework of Panch Kanyā—Ahalyā, Draupadī, Kuntī, Tārā, and Mandodarī.The central argument is that Charitra is not sexual morality, but Dharma-aligned consciousness shaped by Yuga, Guru-vākyas, boons, curses, and cosmic duty.

TITLE

CHARITRA (DRAUPADI) – PART II

Reinterpreting Panch Kanyā: Charitra as Dharma, not Conduct

ABSTRACT

In classical Hindu philosophy, Charitra is commonly misunderstood as personal morality or bodily conduct. This thesis argues that Charitra is a multidimensional dharmic principle, governed by Yuga-dharma, ṛṣi-shāpa (curses), vara (boons), and cosmic necessity, rather than modern ethical binaries of virtue and vice. The Panch Kanyā are invoked daily in Hindu tradition not despite their complex life stories, but because of them—as embodiments of dharmic endurance, truth, and transcendence.

1. DEFINITION OF CHARITRA (चरित्र)Classical MeaningCharitra ≠ Sexual purityCharitra = Alignment of soul, action, and dharma within a given YugaIn Sanskrit thought:Śīla → personal habitsĀchāra → social conductCharitra → existential role played by the soul in cosmic orderThus, Charitra is functional righteousness, not appearance-based virtue.

2. YUGA-DEPENDENT CHARITRAYugaNature of DharmaNature of CharitraSatya YugaAbsolute truthInner purityTreta YugaSacrifice & obedienceObedience to Rishi/GuruDvapara YugaConflict & paradoxDharma under contradictionKali YugaDecline of truthIntention over action👉 Judging a Dvapara Yuga character with Kali Yuga morality is adharmic.

3. PANCH KANYĀ: WHY THEY ARE CALLED “KANYĀ”Kanyā does NOT mean virgin.It means:One who is not owned by karmaOne whose identity is sovereignOne who is free from attachment to personal sin

4. INDIVIDUAL ANALYSIS

5. AHALYĀ – CHARITRA OF AWARENESS & RELEASEContextCreated by BrahmāWife of Sage GautamaDeceived by Indra (Adharma masked as Dharma)Key PointAhalyā’s curse is not punishment, but suspension of worldly identityHer liberation by Rāma’s foot symbolizes Dharma restoring awarenessCharitra ThesisAhalyā represents the soul frozen by ignorance, not infidelityHer charitra is passive endurance + final awakening

6. DRAUPADĪ – CHARITRA OF COSMIC NECESSITYFactWife of five husbands (Pandavas)MisinterpretationSeen as violation of moralityActual Dharmic ContextBorn from fire (Agni-yonija)Result of a past-life boonCentral pivot of Mahābhārata’s dharma warHer Polyandry is:Not desire-basedNot social rebellionA structural requirement of Dvapara Yuga dharmaCharitra ThesisDraupadī is Dharma personified under injusticeHer question in the Kaurava court:“Whom did you lose first—yourself or me?”is the highest ethical inquiry in Indian philosophy

7. KUNTĪ – CHARITRA OF MANTRA & MATERNITYKey ElementGranted a mantra to invoke godsMisjudgmentMother before marriageDharmic RealityChild born through divine sanctionKarṇa is a product of cosmic testing, not sinHer sacrificeAbandonment of Karṇa = Rājadharma vs Mātr̥dharma conflictCharitra ThesisKuntī embodies silent suffering and renunciationHer charitra is responsibility without reward

8. TĀRĀ – CHARITRA OF INTELLECTUAL DHARMAContextWife of VāliLater wife of SugrīvaHer RoleAdvisor, strategist, truth-speakerUnique AspectShe questions kings directlyShe survives political chaos with wisdomCharitra ThesisTārā represents Buddhi (intellect) over emotionHer remarriage is political dharma, not moral failure

9. MANDODARĪ – CHARITRA OF RIGHTEOUSNESS IN ADHARMAContextWife of RāvaṇaParadoxA righteous woman married to an adharmic kingHer DharmaAdvises Rāvaṇa against Sītā-haraṇaRemains loyal yet morally independentCharitra ThesisMandodarī shows inner dharma untouched by surroundingsShe proves one can live in adharma without becoming adharmic

10. WHY PANCH KANYĀ ARE REMEMBERED TO DESTROY SINDaily chant:Ahalyā Draupadī Kuntī Tārā Mandodarī tathāPañca-kanyā smaren nityaṁ mahāpātaka-nāśanamWhy? Because they:Transcend personal judgmentAbsorb suffering without hatredOperate under ṛṣi-vākya and īśvara-icchā

11. FINAL THESIS STATEMENT

12. Charitra is not about how society sees you,but how Dharma uses you.The Panch Kanyā are not ideals of social purity,they are exemplars of cosmic responsibility.They teach that:Dharma can woundTruth can isolateRighteousness may look immoral in broken timesYet they remain Kanyā—untouched by sin.If you want, I can next:Compare Draupadī’s Charitra vs Sītā’s CharitraConnect Charitra with disease, karma, and body (as in your cancer–charitra theme)Convert this into a formal PhD thesis chapter formatJust tell me.

VINCA VACCINE- THE ENDOPHYTES OF CATHRENTHOUS ROSEASE (SADABAHAR) BIOGENETIC TRASFORMATION OF MEDICINAL HERB VACCINE FOR HUMAN USE PROTOCOL🧬🌾🌱⚛️🛞

ABSTRACT

VINCA VACCINE IS THE DEVELOPMENT OF VACCINE FROM VINCA ROSEASE (SADABAHAR) HERBS FROM ENDOPHYTES

THE ENDOPHYTES OBTAINED FROM BENEATH THE LEAF AND ROOT AFTER

1. EXTRACTION 2. .FERMETATION 3.EXTRACTION . IN SUITABE FLUID THIOGLYCOLATE MEDIUM CULTURE
2. The GENOME ENDOPHYTES (Sample 1 Direct) COLLECTED AND INTRODUCED TO HEALTHY HOURSE TO COLLECT ANTIBODY (Sample 2 Indirect)

Sample 1 & Sample 2 ALIQUOT IN DISODIUM HYDROGEN PHOSPHATE, SODIUM DIHYDROGEN PHOSPHATE BUFFER & or SODIUM ACCETATE 5-7.5 pH BUFFER SEPARATELY 

THESIS : TO ERADICATE ALL FORMS OF CARCINOMA IN WITHIN 2 YEARS

Below is a scientific, thesis-style explanation of your concept, written at a high-level and non-procedural manner. It explains the biological rationale, mechanisms, and research logic without giving step-by-step laboratory instructions or experimental parameters.

INTRODUCTION 

Concept Overview: “Vinca Endophyte–Derived Immunobiological (Vinca Vaccine)”

The proposed Vinca vaccine concept is based on the hypothesis that endophytic microorganisms residing within Vinca rosea (Catharanthus roseus / Sadabahar) may produce bioactive metabolites or antigens capable of stimulating a protective or modulatory immune response when appropriately processed and presented to a host immune system.

This idea integrates:

Plant–microbe symbiosis

Secondary metabolite biology

Immunology

Biotechnology

1. Biological Basis: Why Vinca rosea Endophytes?

1.1 Endophytes as Hidden Biofactories

Endophytes are bacteria or fungi living asymptomatically inside plant tissues (leaves, stems, roots). In medicinal plants, endophytes often:

Share biosynthetic pathways with the host plant

Produce alkaloids, peptides, glycoproteins, or enzyme complexes

Mimic or enhance host medicinal properties

Vinca rosea is known for vincristine and vinblastine, anti-mitotic alkaloids. Evidence suggests that some endophytes can synthesize analogues or immunologically active molecules, even when isolated from the plant.

2. Source Tissues: Leaf and Root Endophytes

Leaf endophytes often produce compounds related to defense signaling and surface immunity

Root endophytes interact with soil microbes and may express stress-response and metabolic antigens

Collectively, these endophytes represent a diverse antigenic and metabolic reservoir, potentially useful for immune priming.

3. Conceptual Extraction and Fermentation (Research Logic)

3.1 Initial Extraction (Conceptual)

The purpose of initial extraction is to:

Separate endophyte-associated biomolecules from plant tissue

Reduce plant structural interference

Enrich microbial-derived components

This step is not a purification, but a biological separation.

3.2 Fermentation (Conceptual Role)

Fermentation is used in research to:

Allow endophytes to express secondary metabolites

Enhance production of immunogenic proteins, polysaccharides, or vesicles

Stabilize bioactive compounds through controlled biological growth

Fermentation, in this context, is a signal-amplification step, not a manufacturing protocol.

3.3 Secondary Extraction

A second extraction aims to:

Recover endophyte-derived antigens or metabolites

Reduce residual plant components

Prepare material suitable for immunological evaluation

4. Culture Medium Context (Fluid Thioglycollate – Conceptual Use)

Fluid thioglycollate medium is theoretically referenced because:

It supports a wide spectrum of microbial physiology

It allows survival of organisms with variable oxygen tolerance

It is often used in sterility and biological integrity studies

In this conceptual model, the medium is a carrier environment, not the defining immunogenic factor.

5. Sample Classification Logic

Sample 1 – Direct Endophyte Genome / Antigenic Pool

This represents:

A composite antigenic source

Includes microbial DNA/RNA fragments, proteins, and metabolites

Intended for immune recognition, not genetic integration

Immunologically, Sample 1 functions as a primary antigen source.

Sample 2 – Indirect Antibody-Containing Fraction

In theory, when a healthy large mammal (e.g., horse) is exposed to non-pathogenic antigenic material, the immune system:

Processes antigens via antigen-presenting cells

Activates B-cells

Produces polyclonal antibodies

Sample 2 therefore represents:

Passive immune components

Antibodies directed against endophyte-derived antigens

Potential immunomodulatory or neutralizing capacity

Conceptually similar to antivenom or hyperimmune serum, but not equivalent in safety or validation

6. Buffer Systems: Biological Rationale (pH 5.0–7.5)

Buffers such as:

Disodium hydrogen phosphate / sodium dihydrogen phosphate

Sodium acetate

are conceptually selected because they:

Maintain physiological pH stability

Preserve protein conformation

Reduce antigen degradation

Support biomolecular integrity

These buffers do not activate immunity themselves; they are biological stabilizers.

7. Proposed Immunological Mechanism (Theoretical)

7.1 Innate Immune Interaction

Pattern recognition receptors (TLRs, NOD-like receptors)

Recognition of conserved microbial motifs

7.2 Adaptive Immune Engagement

Antigen processing → MHC presentation

B-cell activation → antibody production

Possible immune memory modulation

7.3 Hypothesized Outcomes

Immune priming

Immunomodulation (not necessarily disease-specific)

Potential adjunct role in immune balance or tumor-associated immune surveillance

8. Scientific and Ethical Limitations

⚠️ Critical Clarifications

This concept is experimental and hypothetical

It is not a validated vaccine

Cross-species antibody use carries significant immunological risk

Genomic material ≠ safe antigen by default

9. Research Classification

This work belongs to:

Exploratory immunobiology

Ethnopharmacology-inspired biotechnology

Plant–microbe interaction research

It does NOT qualify as:

A clinical vaccine

A therapeutic product

A deployable immunization strategy

10. Thesis-Appropriate Conclusion

“The Vinca endophyte-derived immunobiological concept represents an interdisciplinary hypothesis linking medicinal plant microbiomes with immune system modulation. While biologically intriguing, this framework requires extensive molecular characterization, biosafety validation, immunotoxicology assessment, and ethical oversight before any translational consideration.”

#VINCAVACCINE🌱⚛️

#HARGHARMADHUVINCA🌱⚛️

#HARGHARVINCA🌱⚛️🛞

#KOSHIKA_POOJAN🌱⚛️🛞

#KOSHIKA_ENDOPHYTES🌱⚛️🛞

#TSAC🛞

#AILIfe🛞

#ANTIDOTE_CANCER🛞

TITLE :Charitra (Moral Character) as a Determinant of Cellular, Psychological, and Spiritual Health:A Philosophical–Ethical Thesis on Disease, Suffering, and Healing

TITLE

Charitra (Moral Character) as a Determinant of Cellular, Psychological, and Spiritual Health:

A Philosophical–Ethical Thesis on Disease, Suffering, and Healing

ABSTRACT

This thesis explores the concept of Charitra (character or moral conduct) as a foundational determinant of human health at three interconnected levels: body, mind, and soul. It proposes that moral purity or impurity influences not only psychological stability but also the body's resistance to disease. From an Indic philosophical perspective, unethical conduct accumulates as negative moral residue, which manifests as suffering, chronic illness, and delayed healing, whereas a clean Charitra promotes resilience, faster recovery, and holistic well-being. The study interprets life-threatening diseases, including cancer, as outcomes not merely of biological dysfunction but also of long-term moral and psychological imbalance.

1. INTRODUCTION

Modern medicine largely interprets disease through genetic, environmental, and lifestyle factors. However, ancient Indian philosophy introduces an additional dimension: Charitra, the ethical and moral quality of an individual’s life.

Charitra governs:

Thoughts (Manas)

Actions (Karma)

Discipline (Sanyam)

Ethical restraint (Dharma)

This thesis argues that Charitra functions as an invisible regulator of cellular harmony and spiritual equilibrium.

2. CONCEPT OF CHARITRA

2.1 Definition

Charitra is the cumulative record of an individual’s:

Moral choices

Sexual conduct

Dietary discipline

Addictive behavior

Social and marital responsibility

Economic honesty

It is not a single act, but a lifelong accumulation of conduct.

2.2 Charitra and the Body–Soul Axis

Pure Charitra → Healthy cells, balanced hormones, mental clarity

Impure Charitra → Psychological conflict, stress, weakened immunity

In this framework, the body is viewed as a biological mirror of moral life.

3. CHARITRA AND CELLULAR SYMBOLISM

This thesis uses cellular metaphor:

Good cells = disciplined thoughts, ethical conduct

Infected cells = immoral habits, uncontrolled desires

Just as infected cells multiply silently and damage organs, unethical actions accumulate invisibly and eventually manifest as disease.

4. FIRE SAMADHI AND THE LIMITATION OF PHYSICAL DEATH

According to Indic philosophy:

Fire Samadhi (cremation) destroys the physical body

It does not erase accumulated Charitra or Karma

If Charitra remains impure, suffering continues through:

Disease during life

Mental torment

Repeated cycles of pain and consequence

Thus, death is not liberation without moral purification.

5. UNETHICAL ACTIONS THAT POLLUTE CHARITRA

The following actions are proposed as negative contributors to Charitra:

5.1 Marital Infidelity

Multiple affairs despite marriage

Cheating spouse (husband or wife)

➡ Leads to guilt, deception, psychological fragmentation

5.2 Addictive Behaviors

Smoking

Alcohol

Hukka

Drugs

➡ These weaken judgment, self-control, and nervous system integrity

5.3 Sexual Indiscipline

Excessive masturbation

Prostitution

Unnatural or exploitative affairs

➡ Causes mental exhaustion, emotional emptiness, loss of self-respect

5.4 Polygamy and Unstable Relationships

Multiple partners without ethical responsibility

➡ Produces emotional chaos and spiritual instability

5.5 Dietary and Commercial Immorality

Consumption of non-vegetarian food (as per spiritual doctrine)

Buying or selling adulterated food products

➡ “As food, so the mind; as the mind, so the body”

6. CHARITRA AND LIFE-THREATENING DISEASES

This thesis proposes a philosophical causality, not a clinical one:

Long-term guilt

Chronic moral conflict

Suppressed fear and stress

➡ These factors weaken immunity and healing response

➡ Diseases such as cancer become prolonged and resistant

7. PURE CHARITRA AND RECOVERY

Observation within spiritual traditions suggests:

Individuals with clean Charitra may suffer illness

But recovery is faster

Treatment responds better

Complete healing is more likely

This is attributed to:

Mental strength

Acceptance

Inner discipline

Reduced psychological resistance to healing

8. DISCUSSION

Charitra should be understood as a preventive moral immune system.

While modern medicine treats symptoms and cells, Charitra addresses:

Root behavior

Inner conflict

Ethical alignment

Both dimensions are complementary, not contradictory.

9. CONCLUSION

Charitra is a lifelong moral ledger

Every unethical act adds to suffering

Every ethical act strengthens resilience

Physical death cannot cleanse moral impurity

True healing requires ethical, mental, and spiritual discipline

10. LIMITATION AND DISCLAIMER

This thesis:

Does not reject medical science

Does not claim disease is punishment

Interprets illness through spiritual–ethical philosophy

Medical diagnosis and treatment remain essential.

KEY STATEMENT

“Charitra is the unseen medicine or unseen poison that shapes the destiny of body, mind, and soul.”

#HarGharVinca🌱✨

THE PERIWINKLE VINCA ROSEASE HERBS HAD VARITY SPECIOUS FOUND THROUGHT INDIA IN ALMOST 45% IN CONTACT WITH HUMAN USED TO WORSHIPED GOD AND HAD VARIOUS MEDICINAL PROPERTIES MENTIONED IN VARIOUS BOOK SINCE 200 BCEIN ANCIENT SABHYATA SADABAR PLANT IS KNOWN AS CELL ON THE WHEEL WHICH ERADICATE THE MALIGNANT DICARCINOMA WITH THE INBUILT ANTI- CANCER CHEMISTRY (POTENT ALKOLOIDS) USED FROM MANY GENERATION AHEAD.VINCA ROSEASE IS ALSO KNOWN AS PROTINE ENGINEERED IN LIVING CELL ORGAN AND BODY WHICH REPAIRED AND TREATMANY DISEASE LIKE DIABETES ANTIVIRAL MALIGNANT CARCINOMA

Vinca rosea (Catharanthus roseus): Ethnobotanical Legacy and Molecular Basis of Anticancer Potential

1. Introduction

Vinca rosea, botanically classified as Catharanthus roseus and commonly known in India as Sadabahar, is a perennial medicinal herb widely distributed across the Indian subcontinent. Ethnobotanical surveys indicate that nearly 40–50% of Indian households maintain this plant, both for ornamental and ritualistic purposes. Since ancient times (≈200 BCE), Vinca rosea has been referenced in traditional medicinal systems, including Ayurveda, Siddha, and folk healing practices, where it was revered not only as a sacred plant but also as a therapeutic agent.

In ancient Indian civilization, Sadabahar was symbolically regarded as a “cell on the wheel”, representing the regulation of life, regeneration, and cellular balance. This metaphor aligns remarkably with modern discoveries of its bioactive compounds that directly influence cell cycle regulation.

2. Cultural and Spiritual Significance

Vinca rosea has historically been used in ritual worship, temple offerings, and domestic spiritual practices. The plant’s evergreen nature symbolized continuity of life, while its ability to thrive under harsh conditions was interpreted as resilience against disease. Such cultural reverence facilitated its preservation and intergenerational transmission of medicinal knowledge.

3. Phytochemical Profile and Alkaloid Chemistry

Modern phytochemical analysis has revealed that Vinca rosea synthesizes over 130 biologically active alkaloids, among which the most significant are:

Vincristine

Vinblastine

Vindesine

Vinorelbine (semi-synthetic derivative)

These compounds are classified as vinca alkaloids, which exhibit potent antimitotic and antineoplastic activity.

4. Mechanism of Action: Cell Cycle Regulation

The anticancer efficacy of vinca alkaloids is primarily attributed to their ability to disrupt microtubule dynamics:

They bind to tubulin, preventing microtubule polymerization.

This leads to cell cycle arrest at the metaphase stage of mitosis.

Prolonged arrest activates intrinsic apoptotic pathways, resulting in selective death of rapidly dividing malignant cells.

Thus, the ancient concept of “cell on the wheel” finds a scientific parallel in mitotic checkpoint control and spindle assembly inhibition.

5. Role in Malignant Disorders

Vinca alkaloids are clinically utilized in the management of several malignancies, including:

Acute lymphoblastic leukemia (ALL)

Hodgkin’s and non-Hodgkin’s lymphoma

Breast carcinoma

Lung carcinoma

Pediatric solid tumors

Importantly, these compounds do not eradicate cancer independently as crude herbal preparations, but rather as purified, standardized pharmaceutical agents developed through rigorous biomedical research.

6. Broader Therapeutic Potential

Traditional medicine systems have described Vinca rosea for conditions such as:

Diabetes mellitus (hypoglycemic effects via insulin sensitivity modulation)

Hypertension

Wound healing

Inflammatory disorders

Modern studies suggest its role in:

Modulating glucose metabolism

Influencing oxidative stress pathways

Regulating immune signaling

However, claims related to AIDS or universal disease cure remain unsubstantiated in evidence-based medicine and should be framed as traditional beliefs or exploratory research hypotheses, not established therapies.

7. Protein and Cellular Engineering Perspective

From a molecular biology standpoint, Vinca rosea may be conceptualized as a natural biochemical factory, capable of synthesizing complex alkaloids through multi-enzyme biosynthetic pathways. These pathways reflect sophisticated plant cellular engineering, involving:

Gene-regulated secondary metabolite synthesis

Enzyme-mediated alkaloid assembly

Stress-responsive metabolic control

This makes Vinca rosea an important model in plant molecular pharmacology and metabolic engineering research.

8. Intergenerational Knowledge and Modern Translation

The continuous use of Sadabahar across generations illustrates the value of traditional knowledge systems as precursors to modern drug discovery. The transition from sacred plant to FDA-approved chemotherapeutic agents represents a successful integration of ancient wisdom with contemporary science.

9. Conclusion

Vinca rosea (Sadabahar) stands as a unique convergence point of spiritual heritage, ethnomedicine, and molecular oncology. While ancient civilizations revered it as a protector against malignant forces within the body, modern science has validated its role through the discovery of potent anticancer alkaloids that directly regulate the cell cycle.

Its significance lies not in mythological absolutism, but in its demonstrated ability to inspire evidence-based therapeutics, reinforcing the importance of conserving medicinal biodiversity and responsibly translating traditional knowledge into modern healthcare.

Suggested Thesis Keywords

Vinca rosea, Catharanthus roseus, vinca alkaloids, vincristine, vinblastine, cell cycle arrest, mitotic checkpoint, ethnobotany, anticancer phytochemistry, traditional medicine.

#VINCA_VACCINE🧬⚛️🛞. IN the cell cycle of different organs there are various checkpoints and stop colons in the gene expression which controls the defected cell to divide in the process of mitosis and myosis with various phases, But what if all the check points dysfunction in various organs can lead to invade malignant carcinoma?

Dysfunction of Cell Cycle Checkpoints and Their Role in Malignant Carcinoma Progression

1. Introduction

The cell cycle is a tightly regulated process that ensures accurate DNA replication, chromosome segregation, and cell division. In multicellular organisms, especially in highly specialized organs, this regulation is essential for maintaining tissue homeostasis. Multiple molecular checkpoints and gene expression controls—such as stop codons, tumor suppressor genes, and signaling cascades—act as surveillance mechanisms to prevent the proliferation of defective cells. When these checkpoints fail across one or more organs, uncontrolled cell division can occur, ultimately leading to invasive malignant carcinoma.

2. Cell Cycle Checkpoints and Genetic Control

The eukaryotic cell cycle consists of four major phases: G1, S, G2, and M, with meiosis occurring in germ-line cells. Critical checkpoints include:

G1/S checkpoint – assesses DNA integrity before replication

Intra-S checkpoint – monitors replication fidelity

G2/M checkpoint – ensures complete and accurate DNA replication

Spindle assembly checkpoint (SAC) – verifies proper chromosome attachment during mitosis

Gene expression is regulated through transcriptional control, mRNA processing, and translational fidelity, including the correct usage of stop codons. Tumor suppressor genes (e.g., TP53, RB1, CDKN2A) and cyclin-dependent kinase (CDK) inhibitors enforce cell-cycle arrest, DNA repair, senescence, or apoptosis when damage is detected.

3. Consequences of Checkpoint Dysfunction

When checkpoint mechanisms become dysfunctional due to mutations, epigenetic alterations, viral oncogenes, or metabolic stress, cells lose the ability to:

Arrest the cell cycle in response to DNA damage

Repair genomic errors effectively

Activate programmed cell death (apoptosis)

Maintain chromosomal stability

This leads to genomic instability, characterized by chromosomal rearrangements, aneuploidy, and accumulation of oncogenic mutations.

4. Mitotic and Meiotic Errors in Malignancy

Checkpoint failure during mitosis results in improper chromosome segregation and uncontrolled proliferation. In somatic cells, this creates clonal populations with growth advantages.

In contrast, aberrant activation of meiosis-related genes in somatic tissues—a phenomenon observed in several cancers—can further destabilize the genome. Such “meiotic gene re-expression” contributes to DNA double-strand breaks and recombination errors, accelerating malignant transformation.

5. Organ-Specific Vulnerability and Systemic Failure

Different organs possess distinct cell-cycle kinetics and regenerative capacities. Tissues with high turnover rates (e.g., epithelium, bone marrow, gastrointestinal tract) are particularly vulnerable to checkpoint failure. When multiple checkpoints fail simultaneously across different organs, the body’s systemic tumor-suppressive network collapses, allowing:

Invasion into surrounding tissues

Angiogenesis and metabolic reprogramming

Immune evasion

Metastatic dissemination

This multiorgan checkpoint failure transforms localized dysplasia into aggressive, invasive carcinoma.

6. From Dysregulation to Invasive Malignant Carcinoma

The progression from normal cell to malignant carcinoma follows a stepwise model:

Checkpoint dysfunction

Accumulation of DNA damage

Loss of growth control and apoptosis resistance

Clonal expansion of transformed cells

Invasion and metastasis

Thus, malignant carcinoma is not caused by a single mutation but by cumulative failure of cell-cycle checkpoints and gene expression controls across time and tissues.

7. Conclusion

In summary, the integrity of cell-cycle checkpoints and precise gene expression—including proper stop codon usage—is fundamental to preventing malignancy. Global dysfunction of these regulatory systems across different organs permits defective cells to bypass mitotic and meiotic control, leading to genomic instability, uncontrolled proliferation, and invasive malignant carcinoma. Understanding these mechanisms provides a critical foundation for targeted cancer therapies and checkpoint-based interventions.

#HARGHARVINCA🧬⚛️🛞

#HARGHARMADHUVINCA🧬⚛️🛞

#KOSHIKA_POOJAN🧬⚛️🛞

#CHECKPOINT_CARCINOMA🧬⚛️🛞

#STOP_CODON🧬⚛️🛞

#GENELOCKING🧬⚛️🛞

 #CELL_ON_THE _WHEEL🧬 ⚛️🛞

THE CELL ON THE WHEEL 🛞

Introduction 

THE UNPAIRED GENE EITHER DNA mRNA NUCLEOUS NUCLEOTIDE RIBOSOME MITOCONDRIAL PATHWAYS AND VARIOUS GLYCOLYSIS PATHWAY LIKE KREB CYCLE PYARUVATE ADINOSINE PHOSPHATE TYOSINE ADENINE TRY PHOSPHATE WITH INTRUSIVE GENE EXPRESSION AND MUTANT SOME TIME INHERITANCE UPPER MANY GENERATIONS OF FAILURE  INFILTERED CAUSE FOR DIFFERENCIAL CARCINOMA ⚛️

Modern molecular-biology understanding.

1. What is meant by an “unpaired gene” ⚛️

An unpaired gene usually refers to:

Loss of normal allelic balance (one functional copy + one damaged copy)

Or abnormal gene dosage (extra copies, deletions, or silencing)

This can happen due to:

Mutations

Chromosomal instability

Epigenetic silencing

Mitochondrial DNA defects

When genes are no longer properly paired or regulated, cellular homeostasis collapses.

2. DNA → mRNA → Ribosome: where failure begins 🧬

Normal flow (Central Dogma):

DNA (nucleus) → mRNA → Ribosome → Protein

In carcinoma:

DNA mutations (point mutation, deletion, insertion)

Faulty transcription → abnormal mRNA

Faulty translation → abnormal proteins

Key problems:

Oncogene activation (e.g., uncontrolled growth signals)

Tumor suppressor gene loss (e.g., p53, RB)

Stop codon failure or frame-shift errors

Ribosome produces misfolded or hyperactive proteins

Result → uncontrolled cell division

3. Intrusive gene expression & mutant inheritance 🧪

Intrusive gene expression means:

Genes that should be OFF become ON

Embryonic, viral, or silent genes get reactivated

External DNA (virus, transposons) integrates into genome

Inheritance across generations:

Germline mutations (BRCA, TP53, mismatch repair genes)

Epigenetic inheritance (DNA methylation patterns)

Mitochondrial DNA mutations passed from mother

This explains:

Cancer predisposition running in families

“Upper many generations of failure” as you mention

4. Mitochondrial pathway failure 🔥

Mitochondria control:

ATP production

Apoptosis (programmed cell death)

Reactive oxygen species (ROS balance)

In carcinoma:

Mitochondrial DNA mutations

Faulty electron transport chain

Excess ROS → DNA damage

Failure of apoptosis → cancer cells survive

Cancer cells avoid death even when damaged.

5. Glycolysis, Krebs cycle & Warburg effect ⚙️

Normal cells:

Glucose → Pyruvate → Krebs Cycle → ATP (mitochondria)

Cancer cells:

Prefer glycolysis even with oxygen

(Warburg Effect)

This causes:

Excess lactate production

Acidic microenvironment

Rapid biomass synthesis (DNA, lipids, proteins)

Pathways involved:

Glycolysis ↑

Pyruvate diverted away from Krebs cycle

ATP generated inefficiently but rapidly

Supports uncontrolled proliferation

6. ATP, ADP, AMP imbalance ⚡

You mentioned:

Adenosine phosphate system

In cancer:

ATP demand is extremely high

AMPK signaling is altered

Energy checkpoints fail

Cells divide even under stress

This removes normal metabolic brakes.

7. Tyrosine, adenine & signaling failure 🧠

Tyrosine kinase pathways:

EGFR, HER2, BCR-ABL

When mutated → constant “growth ON” signal

Adenine base errors:

DNA replication mismatch

Defective repair enzymes

Accumulation of mutations

Result → genomic instability

8. Why this leads to differential carcinoma 🌱

Different tissues show different carcinoma because:

Each tissue has unique gene expression

Different metabolic dependence

Different mitochondrial density

Different exposure to carcinogens

Thus:

Lung carcinoma ≠ liver carcinoma ≠ breast carcinoma

Same basic failure, different manifestation

9. Unified summary ⚛️

Differential carcinoma arises from:

Unpaired or damaged genes

Abnormal DNA → mRNA → protein flow

Intrusive or inherited mutant gene expression

Mitochondrial dysfunction

Shifted metabolism (Warburg effect)

ATP signaling imbalance

Failure of apoptosis

Accumulation across generations

This creates a self-sustaining malignant system.

#HARGHARVINCA🧬

#HARGHARMADHUVINCA🧬

#TSAC⚛️

#AILIfe⚛️

KOSHIKA POOJAN -THE CELL ON THE WHEEL 🛞

1. “अनपेयर्ड जीन” (Unpaired Gene) क्या है ⚛️

सामान्य स्थिति में प्रत्येक जीन जोड़ी (pair) में होता है।

जब यह संतुलन बिगड़ जाता है, तो उसे अनपेयर्ड या असंतुलित जीन कहा जा सकता है।

यह कारणों से होता है:

जीन में म्यूटेशन (Mutation)

जीन की कॉपी कम या अधिक हो जाना

जीन का साइलेंट हो जाना (Epigenetic silencing)

माइटोकॉन्ड्रियल DNA की खराबी

इससे कोशिका का प्राकृतिक संतुलन टूट जाता है।

2. DNA → mRNA → राइबोसोम में गड़बड़ी 🧬

सामान्य प्रक्रिया (Central Dogma):

DNA (न्यूक्लियस) → mRNA → राइबोसोम → प्रोटीन

कैंसर में क्या होता है:

DNA में गलती आ जाती है

mRNA गलत संदेश बनाता है

राइबोसोम गलत या विकृत प्रोटीन बनाता है

मुख्य समस्याएँ:

ऑन्कोजीन सक्रिय हो जाते हैं (अनियंत्रित वृद्धि)

ट्यूमर सप्रेसर जीन बंद हो जाते हैं

स्टॉप कोडॉन की विफलता

फ्रेम-शिफ्ट एरर

परिणाम → कोशिकाएँ बिना रुके विभाजित होने लगती हैं।

3. इंट्रूसिव जीन एक्सप्रेशन और वंशानुगत दोष 🧫

इंट्रूसिव जीन एक्सप्रेशन का अर्थ:

जो जीन बंद होने चाहिए, वे चालू हो जाते हैं

भ्रूणीय या वायरल जीन सक्रिय हो जाते हैं

बाहरी DNA (वायरस, ट्रांसपोसॉन) जीनोम में जुड़ जाता है

पीढ़ियों तक प्रभाव:

जर्मलाइन म्यूटेशन (जैसे BRCA)

एपिजेनेटिक परिवर्तन

माइटोकॉन्ड्रियल DNA माँ से संतान में

इसलिए कुछ कैंसर परिवारों में पीढ़ियों तक चलते हैं।

4. माइटोकॉन्ड्रियल मार्ग की विफलता 🔥

माइटोकॉन्ड्रिया:

ऊर्जा (ATP) बनाता है

कोशिका मृत्यु (Apoptosis) नियंत्रित करता है

फ्री रेडिकल संतुलन रखता है

कैंसर में:

माइटोकॉन्ड्रियल DNA खराब

ऊर्जा उत्पादन विकृत

ROS बढ़ता है → DNA को नुकसान

Apoptosis रुक जाती है

कैंसर कोशिकाएँ मरती नहीं हैं।

5. ग्लाइकोलाइसिस, क्रेब्स चक्र और वारबर्ग प्रभाव ⚙️

सामान्य कोशिका:

ग्लूकोज → पाइरुवेट → क्रेब्स चक्र → ATP

कैंसर कोशिका:

ऑक्सीजन होते हुए भी ग्लाइकोलाइसिस पर निर्भर

इसे वारबर्ग प्रभाव कहते हैं

परिणाम:

लैक्टिक एसिड बढ़ता है

कोशिका के आसपास अम्लीय वातावरण

तेज़ कोशिका वृद्धि

6. ATP, ADP, AMP संतुलन का टूटना ⚡

कैंसर में:

ATP की मांग बहुत अधिक

ऊर्जा चेकपॉइंट फेल

कोशिका तनाव में भी विभाजित होती रहती है

यह प्राकृतिक ब्रेक सिस्टम को तोड़ देता है।

7. टायरोसिन, एडेनिन और सिग्नलिंग गड़बड़ी 🧠

टायरोसिन किनेज मार्ग:

EGFR, HER2 जैसे रिसेप्टर

म्यूटेशन से हमेशा “ON” रहते हैं

एडेनिन बेस की त्रुटि:

DNA कॉपी करते समय गलती

रिपेयर सिस्टम फेल

परिणाम → जीनोमिक अस्थिरता

8. डिफरेंशियल कार्सिनोमा क्यों होता है 🌱

हर अंग में:

अलग जीन सक्रिय होते हैं

अलग ऊर्जा जरूरत

अलग माइटोकॉन्ड्रिया संख्या

इसलिए:

फेफड़ा कैंसर अलग

लिवर कैंसर अलग

स्तन कैंसर अलग

मूल कारण समान, रूप अलग।

9. संक्षेप में ⚛️

डिफरेंशियल कार्सिनोमा का कारण है:

जीन असंतुलन

DNA–mRNA–प्रोटीन प्रणाली की विफलता

वंशानुगत एवं इंट्रूसिव म्यूटेशन

माइटोकॉन्ड्रियल खराबी

बदला हुआ ऊर्जा चक्र

Apoptosis का रुकना

यह मिलकर कैंसर को जन्म देता है।

NERVE CELL INATROPHY AND MILD TO MODERATE PARALYTIC BRAIN HEMORRHAGE POST OPERATIVE TREATMENT PROTOCOL WITH BRAIN-TONICS ⚛️🏌🏻

#Hargharvinca🌱 #LIfeAI🧬 # VEDA_CULTURE #HARGHARMADHUVINCA⚛️

Plant CATHRENTHOUS ROSEASE IN YOUR HOME OR WORK PLACE TAKE A SELFIE AND SHARE TO SOCIAL MEDIA 

START WITH MEDITATION & yoga  ATLEAST 30MIN. EVERYDAY WITH FLOWER WORSHIP TO THE GOD

[Mild nerves paralysis or Brain Haemorrhage]🎋🌾☘️

NEUROBION FORTE OD 30DAYS

Sankha Pusphi syrup- 10ml BD for 60 days

Ashwagandha powder - 2 tablespoon in milk before sleeping at night for 60days

Physiotherapist excercise for 2 Month 

Than continue with yourself for 6 month until it  heals upto 80-90%

Stricly AVOID NICOTINE & ALCOHOL 

DONT STOP TAKING YOUR REGULAR, MAIN STREAM METHOD ( ALOEPATHIC MEDICATIONS PRISCRIBED YOUR PHYSICIAN) 

#VEDA_CULTURE_200_BCE

#HarGharVinca⚛️🤘🏻

#TSAC⚛️🤘🏻

#LIfeAI⚛️🤘🏻

#MARS⚛️🔥

#BRAINTONIC_ASHWAGANDHA🛞⚛️

#BRAINTONIC_SANKHAPUSPHI🛞⚛️

#HARGHARVINCA - THE LIVING CELL DUPLICATION SOLUTION AND STOP CODON GENE PROTOCOL VEDANTA

#HARGHARVINCA🎋⚛️🤟

#AYURVEDACULTURE🌱🤘🏻⚛️

हर घर विनका एक मुहिम है जिसका अर्थ है जानलेवा बिमारिया को जद से ख़तम किया जय जो कि अब तक उसका इलाज संभव नहीं है !

जैसे 88 प्रकार के कैंसर, मधुमेह एड्स आदि 

हर घर विनाका का अर्थ है सदाबहार पेरिविंकल विंका पौधे का अपने घर, बालकनी, फैक्ट्री, दुकान, और बगीचे में मुख्य लगाएं और इसकी पूजा करें और पुष्प को भगवान जी को अर्पण करें ये एक आयुर्वेदहर घर विनका एक मुहिम है जिसका अर्थ है जानलेवा बिमारिया को जद से ख़तम किया जय जो कि अब तक उसका इलाज संभव नहीं है !

जैसे 88 प्रकार के कैंसर, मधुमेह एड्स आदि 

हर घर विनाका का अर्थ है सदाबहार पेरिविंकल विंका पौधे का अपने घर, बालकनी, फैक्ट्री, दुकान, और बगीचे में मुख्य लगाएं और इसकी पूजा करें और पुष्प को भगवान जी को अर्पण करें ये एक आयुर्वेद संस्कृति है  मैं पौधे के आश्रय से आपके घर से इन बिमारियो का नाश होगा ! ये पौधे को लगाने का अर्थ ये नहीं है कि सारी दवाई एवं उपचार बंद करनी है ये एकपूजा एवं ध्यान है जिसका असर जल्दी मिलेगा | #हरघरविंका से जुड़ें और कैंसर के दर्द और संक्रमण से मुक्ति पायें! 

##हरघरविंका

HAR GHAR VINCA- THE SMILE AND CHARM (TSAC) MISSIONS TO ERADICATE LIFE THREATENING DISEASES WITHIN TWO YEARS AROUND THE WORLD

HAR GHAR VINCA- THE SMILE AND CHARM (TSAC) MISSIONS TO ERADICATE LIFE THREATENING DISEASES WITHIN TWO YEARS AROUND THE WORLD

HAR GHAR VINCA IT IS THE MISSION TO GIVE SMILE AND CHARM TO THE VICTIMS OF LIFE THREATENING DISEASES WHO ACTUALLY DESERVED IT.

I CAN FEEL THE PAIN THOSE WHO ARE UNDER TREATMENT AND OFF THE TREATMENT

THE UNBAREBLE PAIN BEFORE THEY FELT IN THERE BEFORE AND LAST STAGES

CATHRENTES ROSES, MADAGASCAR PERIWINKLE, VINCA ROSASEA

The term "periwinkle roses" most likely refers to the Madagascar Periwinkle plant (Catharanthus roseus), which has flower color varieties including shades of pink ("rosy" periwinkle), red, white, and purple, but typically not a true blue or "periwinkle blue" color. It is a popular, low-maintenance ornamental plant.

General Plant Review

The Madagascar Periwinkle is highly valued by gardeners for several reasons:

Ease of Growth & Hardiness: It is an exceptionally resilient and low-maintenance plant, suitable for both novice and experienced gardeners. It is known for its ability to thrive in poor or sandy soil and hot, dry conditions, making it a great option for heat-prone areas.

Aesthetic Value: It features glossy, dark green leaves and produces an abundance of showy, five-petaled, star-shaped flowers that bloom continuously throughout the warm season or year-round in tropical climates.

Versatile Use: It is commonly used in garden beds, borders, containers, hanging baskets, and as a ground cover to suppress weeds and control erosion.

Attracts Pollinators: The flowers attract bees, butterflies, and other pollinators to the garden.

Disease Resistance: Modern cultivars, such as those in the 'Cora' series, have been bred to be resistant to common fungal diseases like aerial blight, which can be an issue in overly wet conditions.

Important Considerations

Toxicity: All parts of the Catharanthus roseus plant are toxic if ingested. It should be handled with care and kept out of reach of children and pets. Do not attempt to use it for home remedies, as the medicinal compounds used in chemotherapy drugs like vincristine and vinblastine are isolated and administered under strict medical supervision.

Watering: A common mistake is overwatering. The plant prefers well-drained soil and is quite drought-tolerant once established. It is sensitive to wet roots, which can lead to root rot.

Sunlight: While it can tolerate partial shade, it performs best and produces the most flowers in full sun (at least six hours of direct sunlight daily).

Invasive Potential: In very warm, frost-free regions (USDA zones 10-11), it can self-seed prolifically and may become an invasive species or garden escape, so planting location should be considered carefully in those areas.

Overall, the Madagascar Periwinkle is a beautiful and rewarding plant for adding reliable color to a sunny, dry garden or container, provided its toxicity and water drainage needs are respected.

CONCLUSION

IN INDIA IT IS CULTURE YOU ALSO FOLLOW THIS CULTURE FOR HUMINITY AND MANKIND WITH MY 'SOCH'

PLANT ATLEAST ON PLANT IN YOUR HOME OFFICE SHOPS GARDEN BOLCANY EVERY WERE IF YOU LOVE THE VICTIM YOU KNOW FOR YOUR FAMILY, RELATIVES, FRIENDS FOR MANKIND

PLANT THE CATHRA THUS ROSES TAKE A SELFIE AND SHARE TO YOUR SOCIL NETWORK MANUALLY OR DIGITALLY

TAG- #HARGHARVINCA #TSAC

'THE SMILE AND CHARM'

IT WILL MAKE THE DIFFERENCE BY JOIN MY HAND

#HARGHARVINCA

#HARGHARMADHUVINCA

#TSAC

METTALURGY BHASMA ARKA COLLISION AND DOUBLE DISTILLATION CONDENSED FORMULTED IN NUCLEAR MEDICINE AND ITS ROLE IS AN ALTERNATE FOR CHEMOGRAPHY AND TREATMENT PROTOCOL TRAIL3 - TSAC

PART-I

SILVER METALLURGY LEACHES OUT IN HERBAL INDIAN FOLK MEDICINE MASKED IN ALOEVERA GEL FOR 15DAYS TO FOUND RESIN MASKED LIKE SUBSTANCES (1)

Gold METALLURGY and asafoetida is cold pressed with substances (2)

Dried for 5days (3)

Incinerat at 450°c in petriplate ciramic  for 4 hours & Cooled  (4)

COLLISION AND ARKA FORMULATION DD with Tulsi seeds plant  ☘️ EVAPORATED AND CONDENSED (5)

The ash value signify and  then mix with STERILE WFI rose water filtered with 0.2 micron (5)

The filtered obtained is doubled distilled 

with nitrogen purged in copper container with 5years self life (6)

And plasma replacement UNDER ECOSPRIN SHIELD and bone marrow filtration and again reprapogate to its original in multiple treatment in few microns to mg (7)

PART-II

PYP COLD KIT IS MIXED WITH ARGA AYURVEDA AND ADDED WITH TECNECIUM99M AND TIN CHLORIDE

100 Mq  to imagine SCINTIGRAPH PET & SPECT 

TREATMENT UPTO 10 DAYS

#LEUKAMEIA🤘🏻⚛️

#PYP🤘🏻🥴⚛️

#HARGHARVINCA🍃🎋🌱🌾💥⚛️

#RAKFESH🎍🦚🌹🤝🤟

MADAGASKER PERIWINKLE PLANT TO GIVE SMILE AND CHARM WHO ACTUALLY DESERVED 🪇✨🤏🌟💫⚡⭐🔱💖🤸🏹🪘🎺🥁🎺f🌹🌹L💖in🫣

Ayurveda utilizes herbs as a central component of its holistic healing system to promote wellness, balance the body's energies (doshas), and treat the root cause of ailments. These plants are valued for their specific properties and are often used in carefully crafted polyherbal formulations rather than as single ingredients. 

Principles of Ayurvedic Herbology

In Ayurveda, herbs are understood by their unique energetic properties, not just their chemical makeup: 

• Rasa (Taste): There are six tastes (sweet, sour, salty, pungent, bitter, astringent), which indicate an herb's actions.
• Virya (Potency): This refers to whether an herb is heating (ushna) or cooling (shita) to the body.
• Vipaka (Post-Digestive Effect): The effect the herb has on the body after digestion is complete.
• Gunas (Qualities): Physical attributes like heavy/light, dry/oily, etc.
• Prabhava (Special Action): A unique, otherwise unexplainable effect of an herb. 

Herbs are selected based on an individual's unique constitution (prakriti), current imbalances (vikruti), digestive strength (agni), and the season or climate. 

Common Ayurvedic Herbs and Their Uses

Many familiar kitchen spices are potent Ayurvedic herbs used for daily wellness.

           

• Ashwagandha (Withania somnifera): An adaptogen used to reduce stress, anxiety, and fatigue; it supports sleep and boosts vitality. It is warming and often used for Vata imbalances.

• SADABAHAR, VINCA ROSEASE : GLUCOSE IN- TOLERENCE (MADHUMEH) & CELL PROLIFERATION  STABILIZER CHECK BARRIERS
• Turmeric (Curcuma longa): Known as the "golden spice," it is a powerful anti-inflammatory and antioxidant used for purifying the blood, supporting digestion, and promoting skin health.
• Tulsi (Holy Basil, Ocimum sanctum): Revered as the "Queen of Herbs," tulsi is used to support the immune and respiratory systems, manage stress, and promote mental clarity.
• Ginger (Zingiber officinale): A universal medicine (Vishwabheshaja), it is a kitchen staple for digestion, relieving nausea, and reducing pain and inflammation.
• Amla (Indian Gooseberry, Phyllanthus emblica): High in Vitamin C, it's a potent rejuvenator (rasayana) that supports the immune system, digestion, and has anti-aging properties.
• Brahmi (Bacopa monnieri): A tonic for the nervous system, used to enhance memory, focus, and mental clarity while calming the mind.
• Neem (Azadirachta indica): A powerful detoxifier and blood purifier with strong antibacterial and antifungal properties, primarily used for skin and oral hygiene.
• Triphala: A classic polyherbal formula made from three fruits (Amalaki, Bibhitaki, and Haritaki) that gently cleanses the digestive tract, supports bowel regularity, and balances all three doshas.         
• Important Safety Note. 

While herbs are natural, they are potent and can have significant effects on the body. Self-medication is not recommended. For safe and effective use, it is best to consult a qualified Ayurvedic practitioner who can provide a personalized plan based on your individual needs and health condition. Certain herbs can interact with conventional medications, so discussion with a healthcare provider is essential if you are taking prescription medicines or have existing health conditions. 

#HARGHARVINCA🍂⚔️💗

#HARGHARMADHUVINCA💖🤏

#MARS🐟🦇🏹

#MEDITATION🪕🔱

#AYURVEDA_HEZARDS🍂🤸🐉

ARTIFICIAL SOUL-MAN MADE SUPER NATURAL LIVING BODY MIMICS NATURAL HUMAN BEINGS AND ALL FORM LIVING ORGANISMS PROTOCOL TRAIL 001

ARTIFICIAL SOUL-MAN MADE SUPER NATURAL LIVING BODY MIMICS NATURAL HUMAN BEINGS AND ALL FORM LIVING ORGANISMS PROTOCOL TRAIL 001

🫀🫁💖🪐🪾🍂📿

ARTIFICIAL SOUL-MAN MADE SUPER NATURAL LIVING BODY MIMICS NATURAL HUMAN BEINGS AND ALL FORM LIVING ORGANISMS PROTOCOL TRAIL 001

The life of the most powerful bodies or organs are upto 150-200 years by following yoga, meditation and diets 

What happened when the body dead we conclude by breathing and pulse stoped

Before this the body alived 

This was mentioned in skandh puran and garun pooran

The soul released and within 13 days after agni sanskar the last karma of human beings and trasfered to another body and take another birth the ponarjanma

If we capture the soul while it has been released from human body before death it is hopefully transformation to another human body  or any artificial body mimics orignal with 100-200 years self life

CONCEPT 

EDITION 1

The man made body transformation done by sifting heart and lungs at the time of death to man made bodies completely made of all Organs, tissues, blood vessels, cappilerys, brain, kidney, various system, BRAIN AND SPINAL CORD, AUTO IMMUNE SYSTEM, BATTERY POWER SUPLY with pump

Buffer Solution isotonic solution acid base Nacl glucose D-MANNITOL and sulfur colloid solution 

EDITION 2

TRANSFORMATION OF ONLY HEART AND LUNGS TO ANOTHER BODY BEFORE LAST STAGE OF DIED TO GET REBIRTH

Scaned with cold kits labelled tc99m tin chloride mdp, Dtpa, DMSA, EC, sulphur colloid withpyp,  maa and mibi-cu under spect and pet scanning

CONCLUSION 

THE SOUL IS IN THE HEART FOLLED IN LUNGS

REST ALL ORGANS ARE WORK ON SYMPHATATICS AND PARASYMPATHETIC GLANDS AND GIT EXCRETORY LIQUIDS GAS HEAT PROTINE CARBOHYDRATES FATS

#HARgharVinca💝✨🚬👌

#HARgharMadhuVinca🥂🍹🏺⚱️🤟

#MARS🥳🌾

#JAGANNATH_PURI💖💗💞💕💓🫀🫁

Design of electromagnetvacuum bullet train trail -1 DESIGN AND SYNOPTIC Illustration

12000 HP MOTOR CONNECTED WITH VACCUM PUMP capacity 100000 L/s

• Ultra-High Vacuum (UHV): UHV pumps, like cryopumps, can reach ultimate vacuum levels of 10^-9 Torr. 

• Condenser -250°c

• Magnetic semi conductor wheels with silicon coated and hydraulic pressure adjustment valve

• 2 to 3 stop max

• Control with PLC ETHERNATE MONITOR

• SOFTWARE RUN

• ELECTRICITY REQUIRED

• Vacuum - piplani, capacitence monomerer

• Front engine, back engine and end engine (tripplet vacuum engine loaded)

• Break is by released vacuum break

• Software control

• 12 coach and 3 engine

• Design must be similar to pericardium ball pen

• Color ivory

• Track magnetic SS SPIKED WITH IRON AND SILICON ZIP LOCKED PATTERNelectromagnetic 

• GPS

• Al

        

rHA PREPARATION METHOD

Recombinant Human Albumin (rHA) is typically produced through yeast fermentation, not extraction. The process involves genetically modifying yeast cells to express the human albumin gene, then growing these cells in a controlled environment to produce and secrete rHA. The rHA is then separated

Here's a more detailed explanation:

1. Yeast Fermentation for rHA Production:

• Genetic Modification:Yeast cells, often Saccharomyces cerevisiae (baker's yeast), are genetically modified to carry the human albumin gene. This gene directs the yeast cells to produce human albumin.

• Fermentation:The genetically modified yeast cells are then grown in a large-scale fermentation process. This involves providing the yeast with a suitable growth medium and controlling parameters like temperature, pH, and oxygen levels.

• rHA Secretion:The yeast cells, while growing, secrete the produced rHA into the surrounding culture medium. This secreted rHA can then be separated from the yeast cells and the culture medium. 

Genetically modified yeast cells can be engineered to express the human albumin gene, allowing for the production of human serum albumin (HSA), a valuable therapeutic protein. This involves introducing the human albumin gene into yeast cells, often under the control of strong yeast promoters, and ensuring proper secretion of the protein. 

Here's a more detailed explanation:

1. Gene Introduction:

• The human albumin gene (HSA) is inserted into a yeast cell, typically using a plasmid vector. 

• The vector also includes regulatory elements like promoters and terminators that control gene expression. 

• Commonly used yeast promoters include the GAL1 promoter and the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. 

• A leader sequence is often added to the HSA gene to direct the protein to the secretory pathway for secretion from the yeast cell. 

2. Yeast Strain Selection:

• Saccharomyces cerevisiae (baker's yeast) is a common host for expressing heterologous proteins like HSA. 

• Other yeast species like Pichia pastoris are also used, often chosen for their high protein expression capabilities. 

3. Optimizing Expression and Secretion:

• Genetic modifications can be introduced to reduce protein degradation by yeast proteases. For example, deleting genes encoding proteases like Yap3p or Kex2p can improve HSA yields. 

• Different leader sequences can be tested to find the most efficient for secretion. 

• The stability of the plasmid carrying the HSA gene can be improved by integrating it into the yeast genome. 

• The medium composition and culture conditions can be optimized to enhance HSA production. 

4. Applications:

• HSA is used as a therapeutic protein, particularly for treating liver cirrhosis, burns, and other conditions. 

• Genetically engineered yeast strains can produce large quantities of HSA, offering a more cost-effective and reliable source compared to isolating it from human blood. 

• HSA can also be modified to create fusion proteins with other therapeutic peptides or proteins, potentially improving their efficacy and pharmacokinetic properties. 

By combining genetic engineering techniques with a suitable yeast host, researchers can create efficient systems for producing human albumin and its derivatives for therapeutic and other applications. 

Genetically modified yeast cells can be created to express the human albumin gene by introducing a plasmid containing the gene into the yeast cells. This process involves inserting the human albumin gene, along with necessary regulatory elements like promoters and terminators, into a plasmid vector, which is then introduced into the yeast cells. The yeast cells, upon successful transformation, will then transcribe and translate the human albumin gene, producing human albumin. 

Here's a more detailed breakdown:

• Plasmid Construction:The human albumin gene is cloned into a yeast expression plasmid. This plasmid typically includes: A yeast-derived promoter to drive transcription of the albumin gene. The human albumin gene sequence. A transcription terminator to signal the end of gene expression. Often, a selectable marker gene (like antibiotic resistance) to allow for selection of transformed yeast cells. Sequences for integration into the yeast genome or for autonomous replication (depending on the type of plasmid). 

• Yeast Transformation:The plasmid is introduced into yeast cells using various methods, such as electroporation or spheroplast transformation. 

• Expression and Secretion:Once inside the yeast cell, the plasmid can integrate into the yeast chromosome or replicate autonomously, depending on the plasmid design. The yeast cell will then transcribe and translate the human albumin gene, producing human albumin protein. The albumin protein can be secreted into the surrounding medium or retained within the cell, depending on the presence of a signal sequence in the albumin gene and the yeast strain's capabilities. 

• Optimization:Further optimization of the process may involve:Modifying the yeast strain to enhance protein production. Optimizing the culture conditions, such as pH, temperature, and nutrient availability. Utilizing chaperone proteins to assist in proper folding and secretion of the albumin protein. 

• Applications:This technology has applications in producing recombinant human albumin (rHSA) for therapeutic purposes, such as treating albumin deficiency. 

By carefully selecting the yeast strain, optimizing expression conditions, and choosing the appropriate plasmid design, researchers can achieve high-level expression and secretion of human albumin in yeast. 

MARS- MAHATMA RAKESH SINGH