Study of Alzheimer's and Demantia of Datura Metal L. Plant extracts in transgenic mice and swiss albino mice activity behavioral patterns

 Elevated plus maze (EPM) test

Procedure

The elevated plus-maze consists of two open arms,

43×15cm (L×W), and two enclosed arms, 43×15×23cm

(L×W×H), opened to the top, arranged in such way that the

two open arms are faced opposite to each other. The maze

is elevated to a height of 70cm.10

After one hour of oral administration of the test drug or the

standard, the rat is placed at the centre of the maze, facing

towards one of the enclosed arms. After 5min of 

observation the following parameters are noted

• The number of entries into open arm and closed arms 

• Time spent in the open and enclosed arms 

Digital Actophotometer

Procedure

A continuous beam of light from six lights will be made to

fall on corresponding photoelectric cells, the photoelectric

cells will get activated when an animal crosses the beam

of light and thereby cuts off (crossing) the rays of light

falling on it. These crossings are counted automatically for a aperiod of 10 min by the machine. The number of cuts off

was taken as a parameter of the locomotor activity of the rats

5 rats are placed in the digital actophotometer 1 hr afterr Drug administration and number of crossings are counted

for a period of 10 min 

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