Hepatoprotactive activity of Ethanolic extract of mentha (Mint) leaves in paracetamol-induced liver damaged in Wistar albino rats

 Parameters

SGOT

SGOT

ALP

LIVER FUNCTION TEST

SERUM BILIRUBIN TEST

BILE DUCT CANNULATION

URINE TEST

ULTRASOUND

 

Acute toxicity

An acute oral toxicity study was performed as per OECD guidelines for the testing of chemicals, Test No. 423 (OECD; acute oral toxicity-acute toxic class method). Swiss albino mice (n = 6) were used for the acute toxicity study. The animals were kept overnight with access to water but not food, after which the mentha extract was administered orally at a dose level of 500, 1000 and 2000 mg/kg body weight and the animals were observed for 24 h. Further, they were observed continuously for the first 2 h for morbidity and up to 24 h for mortality. If mortality was observed in 2 out of 3 animals, then the dose administered was identified as a toxic dose. If mortality was observed in one animal, then the same dose was repeated again to confirm the toxic dose. If mortality was observed again, the procedure was repeated for lower doses (300, 50 and 5 mg/kg body weight).

Experimental design for hematological and hepatoprotective study

A total of 35 Sprague-Dawley male rats (Rattus norvegicus) were randomly divided into five groups conveying seven animals of each group and were kept in the experimental period of 14 days, as follows:

1. Vehicle

Animals received 0.05% tween 80 dissolved in 0.9% NaCl solution at 0.5 mL/rat with normal diet.

2. Negative control (Paracetamol)

Animals received paracetamol alone at 640 mg/kg BW (p.o.) dissolved in the vehicle. 

3. Treatment 1 (Mentha50 mg/kg)

Animals received mentha at a dose of 50 mg/kg BW (p.o.) and paracetamol (640 mg/kg BW, p.o.) dissolved in the vehicle.

4. Treatment2 (Mentha 100 mg/kg)

Animals received mentha at a dose of 10 mg/kg BW (p.o.) and paracetamol (640 mg/kg BW, p.o.) dissolved in the vehicle.

5. Standard(Silymarin 50 mg/kg)

Animals received silymarin at a dose of 50 mg/kg BW (p.o.) and paracetamol (640 mg/kg BW, p.o.) dissolved in the vehicle.

#Rakfeshhepatoprotective

#Rakfeshmentha

#pharmareserchprotocol

#pharmascienceanddevelopment

EVALUATION OF ANTI-INFLAMMATORY ACTION OF AQUAOUS AND ETANOLIC EXTRACT OF THE LEAVES OF CALOTROPIS GIGANTEA IN CARRAGEENAN INDUCED RAT PAW EDEMA IN ALBINO RATS

 Carrageenan-induced rat paw edema model

The rats were divided into eight groups (n = 6), each receiving distilled water (control), diclofenac 20 mg/kg p.o. (reference standard), and 50, 100, 200 mg/kg p.o. dose of the AE and EE of C. Gigantea  respectively. Carrageenan (0.1 mL of 1%) was injected into the subplantar tissue of the right hind-paw of each rat. The volume of the carrageenan injected into the foot was measured at 0, 30, 60, 120, and 180 minutes using a plethysmometer (Biodevices, New Delhi, India). The percentage inhibition (PI) at each time interval was calculated$\text{PI}=\frac{{(\text{V}}_{\text{t}}-{\text{V}}_{\text{0}})\text{control}-({\text{V}}_{\text{t}}-{\text{V}}_{\text{0}})\text{treated}}{({\text{V}}_{\text{t}}-{\text{V}}_{\text{0}})\text{control}}\times \mathrm{100PI}$

PI= (Vt-V0) Control - (Vt-V0)Teatment*100

Upon

(Vt-V0) Control

V0 = Mean paw volume at 0 hours

Vt = Mean paw volume at a particular time interval

Ref :Vogel

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EFFECT OF POLYHERBS CONVOVULUS PROSTRATUS, BACOPA MONNIERI AND CANNABINOIDS IN ALZHEIMER'S TRANSGENIC MICE

The commonly used experimental animal models are transgenic mice that overexpress human genes associated with familial AD (FAD) that result in the formation of amyloid plaques. 

Alzheimer's is defined by the presence and interplay of both amyloid plaques and neurofibrillary tangle pathology 

Alzheimer's disease is a progressive neurologic disorder that causes the brain to shrink (atrophy) and brain cells to die. 

Alzheimer's disease is the most common cause of dementia continuous decline in thinking, behavioral and social skills that has effect on person's ability to function independently.

AD -Alzheimers 

Ref-vogel

Effect of Ocimum sanctum (OS) leaf extract and Endophytes on hepatotoxicity induced by antitubercular drugs in albino rats

Parameters:

AST

ALT

Serum bilirubin test

Serum albumin test

Liver biopsy

Ultrasound